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– Extracellular vesicles (EVs) derived from 3D mesenchymal stem cell (MSC) cultures offer a significant advantage over traditional 2D systems due to their higher yield, enhanced bioactivity, and therapeutic potency. The 3D culture environment closely replicates native tissue conditions, leading to improved EV production, optimized cargo composition (e.g., enriched proteins and RNAs), and superior regenerative and immunomodulatory effects.